Selling Fertilizer for Plankton Culturing

[xxxsoulxxx]

Hi,

Selling 2 kind of fertilizer for culturing of phytoplankton:

1) Urea: (NH2)2CO

2) Mono-Potassium Phosphate: (KH2PO4)

Phytoplankton culturing requires 3 main kind of macro-nutrients : Nitrogen, Potassium, Phosphate. Also required are micro-nutrients (could be purchased at any marine shops, trace minerals)

Recommended dosage ratio is 5:1 (nitrogen:phosphate) (best to test for nitrate and phosphate before harvesting and dosing into tank)

Selling both in crystal form (soluable in water), in tao huay box, $15 for Urea and $30 for mono-potassium phosphate.

[FuEl]

There is more to culturing marine phytoplankton than just pure N,P, K.

Industry standard has been guillard's f/2 medium. Probably better for aquarium.

https://ccmp.bigelow.org/node/79

[xxxsoulxxx]

There is more to culturing marine phytoplankton than just pure N,P, K.

Industry standard has been guillard's f/2 medium. Probably better for aquarium.

https://ccmp.bigelow.org/node/79

Hi Fuel,

The formula for guillard's is below:

1) NaNO3 (Sodium Nitrate)

2) NaH2PO4 H2O(Sodium Phosphate in water form)

3) Na2SiO3 9H2O (Sodium Silicate in water form) (Have to ignore this as it's for diatoms culture not plankton)

4) Trace & Vitamin

I'm no phd in chemistry but i've done some research and i believe it can be subsituted with the below:

1) NaNO3 (Sodium Nitrate) -> Urea: (NH2)2CO

2) NaH2PO4 H2O(Sodium Phosphate in water form) -> Mono-Potassium Phosphate: (KH2PO4)

3) Na2SiO3 9H2O (Sodium Silicate in water form) (Have to ignore this as it's for growing diatoms not plankton)

4) Trace & Vitamin -> i purchased a seachem trace and vitamin which contains all the ingredients

The reason for subsitution is because Sodium(Na) could be found in salt (Sodium Cloride), Hydrogen (H) and Oxygen (O) makes up water (H2O), Carbon © and Oxygen (O) makes up carbon dioxide (CO2). Potassium (K), i last checked is not harmful in anyway even in large quantity and is actually required for all plants in the world.

Plankton culture is therefore limited to only nitrate, phosphate, trace and vitamin.

http://en.wikipedia.org/wiki/Urea

Urea: "In the soil, it hydrolyses back to ammonia and carbon dioxide. The ammonia is oxidized by bacteria in the soil to nitrate which can be absorbed by the plants"

http://en.wikipedia.org/wiki/Monopotassium_phosphate

What's good about dosing separately is that you can control which of the ingrdients u want in, nitrate, phosphate or trace and vitamin. Normally, due to the external factors eg lighting, temperature, ph, plankton will not consume all the ingredients in equal ratio. Nitrate will often nitrify, resulting in extra phosphate but lack of nitrate. To dose guillard will be adding phosphate and nitrate causing phosphate buildup.

Thanks.