It's best that waterflow thru a DSB is kept to the minimum as possible. This is the reason why for remote DSB in sumps, water flows OVER the DSB...not filtering thru a DSB. If water movement thru a DSB is rapid, oxygen concentration in the DSB would be higher, leading to little or even no anoxic zone for anaerobic bacteria to grow, impeding denitrification.
If water movement thru a DSB is slow, it would be best, as less oxygen enters the DSB, enabling an anoxic zone to form for anaerobic bacteria to form.
Using a U.G would render the DSB useless as fresh oxygen will oxygenate the DSB, enabling only aerobic bacteria to form, causing nitrate buildup over time.
Actually...IMO..a DSB works better in tropical climates due to lower levels of dissolved oxygen levels in the water and due to higher metabolic rates of organisms. Less dissolved oxygen would thus enter a DSB, making it possible for a large anoxic zone to be formed.
Anaerobic bacteria use sulphur as they break down nitrates into nitrogen gas. The biproduct of this process would be hydrogen sulphide which could build up in the DSB. If levels of hydrogen sulphide is high, disturbance of the DSB could release hydrogen sulphide into the water, causing a rapid drop in pH, and causing inhabitants to die due to pH shock.
Hope I am speaking sense...