Bro, I did a search before doing the test. No one is doing it with a 3L water volume?
Why the sudden jump from vinegar to vitamin c? Acetic and citric acid is not ascorbic acid. 5% of acetic acid will cause a pH of 2? Even when I diluted it to 0.005%? Acetic acid is an organic acid. Most organic acids are weak acids; they do not disassociate completely in the presence of water. Commercial distilled vinegar found in supermarket has a pH ranging from 2.4-3.4. Vitamin C will degrade as soon as it touches water? Either the lemon is very dry or the doctors and nutritionists lied about lemons having vitamin c. One may say the preservatives found in lemon is critic acid, that I agree. If critic acid is that unstable, the vitamin c in lemon will be degraded within 12 hours after it is grown?
If the acid is degraded, how is it going to affect the pH of the tank water?
I agree there is a certain limit of the amount of bacteria that the water can hold. If you see very matured sewage water, that's roughly the amount. I can safely say the visibility through the water is less than a few cm and no higher lifeforms can really exist in it. If that's the case, all my fishes would have flipped?
I see no reason why one cannot revisit something that has been done before. It is through the revisitings that numerous discoveries were remade and corrected. Revisitings also allow for verification of results. This methodology is called science?
Dosing sodium ascorbate can be dangerous in a nano tank due to the increase in sodium ions which may increase salinity.
One can say this can be a cause, that can be a cause, even a fart a thousand miles away could have caused the failure (butterfly effect?) By doing this and comparing the results with others, we may find the exact cause and maybe come up with a solution. Imagine how many nano tanks one can save from the hands of numerous new reefers.
Anyway, someone is intending to take fishes to space and they most probably won't release to the wild or return them to earth.
Going down the same road reinforces the path. Eventually, it will create a path that leads to results. Try telling the ants not to do that?
As for the results, mine differs from yours in that my fishes were not even stressed out. My protein skimmer remains normal, there's no excessive skim-mate and the water is very clear. It is very strange that only mushroom corals were affected. All the other non-photosynthetic lifeforms were not visibly affected. The coralline algae still looks purplish and reddish.